This paper demonstrates a low cost, scalable chemiresistive nanobiosensor platform for the label-free detection of DNA hybridization. The platform comprises electrospun PANi/PEO nanofibers precisely aligned between two conductive electrodes of a microelectrode array. The aligned fiber is functionalized with probe-DNA. The change in the electrical conductivity upon target nucleotide hybridization is used as the transduction principle. The efficiency of this hybridization detection platform is demonstrated using detection of dengue virus specific consensus primers in the concentration range of 1 pM-1 μM. © 2017 IEEE.