Synergistic effect of different fibers on the improvement of fracture behavior of concrete is a widely accepted phenomenon. However, the fracture mechanisms behind their improvement need to be clearly understood for optimizing the fiber dosage. In this work, the fracture behavior of synthetic fiber-reinforced concrete (SynFRC), steel fiber reinforced concrete (SFRC) and hybrid fiber reinforced concrete (HFRC) under flexural loading is studied using acoustic emission (AE) technique. Three different fiber dosages, by volume of concrete, of 0.50%, 0.75% and 1.0% and their hybrid combinations were considered as study parameters. Test results show that it is possible to retain sufficient workability in the fresh state and obtain good fracture resistance through a hybrid combination of hooked end steel fibers and macro- synthetic polyolefin fibers. AE parameters were investigated to acknowledge the efficiency of the fibers in improving the fracture behavior and toughness of SynFRC, SFRC, and HFRC. AE parameters such as hits, events, AE energy, and 3D-crack source locations were presented to illustrate the role of different fiber dosages on the Mode-I fracture response. The source locations of AE event above the artificial notches created at the mid-span of specimens were investigated and classified into tensile or shear cracks. AE and fracture energy increased with fiber dosage. SFRC demonstrated higher energy dissipation capacity followed by HFRC and SynFRC. © 2018 Elsevier Ltd