This paper presents a simple process of bio- functionalizing the surface of UV crosslinked SU-8 surfaces. SU-8 is a popular photo-patternable epoxy based polymer used extensively in Lab-on-a-Chip and MEMS applications. In this work we realized a SU-8 surface with Hydroxyl (-OH) groups by adding a copolymer Glycidol. Biotin Hydrazide is immobilized over this surface after APTES and Glutaraldehyde treatment. FTIR spectroscopy analyses were performed on the samples at various stages of bio-functionalization process. Copyright 2015 by TechConnect. All rights reserved.