The prediction of transmission loss characteristics of a short chamber requires the study of both longitudinal as well as transverse modes. This prompts the development of a three-dimensional (3D) transfer matrix, which can address the wave propagation in all three directions. Accordingly, an analytical methodology for transmission loss characteristics of an annular cavity using Green's function method is presented in this paper. The 3D wave equation is used to derive the mode shape functions for the cavity. The average pressure on the inlet and outlet ports is predicted using the Green's function derived from the mode shapes. Three different port location configurations of the annular cavity are considered for establishing the analytical methodology, viz., face-inlet-face-outlet (FIFO), side-inlet-side-outlet (SISO), and face-inlet-side-outlet (FISO). Numerical model results are used to corroborate the analytical results. The effects of geometrical parameters on the transmission loss are studied. © 2017 Acoustical Society of America.