The real time monitoring of gold nanoparticles (AuNPs) deposition on a modified surface is experimentally investigated. Reflectance of the substrate is used to monitor the deposition of during the deposition of AuNPs within the microfluidic chamber. The reflectance is strongly dependent on the particle density at a given point and exhibits both dips and peaks. The assembly kinetics are compared for both modified and un-modified surfaces. It is shown that the assembly kinetics can be increased significantly due to the presence of appropriate charge species due to the surface modification. © 2022 IEEE.