Water-based ferrofluids of substituted ferrites Fe1-x Mnx Fe2 O4 (0≤x≤1) have been prepared by the co-precipitation method and characterized. Particles are superparamagnetic with an average particle size of about 10-12 nm. The specific absorption rate (SAR) was measured by calorimetric measurement at a frequency of 300 kHz and a field of 10-45 kAm. The variation of SAR and magnetization of Fe1-x Mnx Fe2 O4 with Mn concentration shows similar dependence. Innocuousness studies of Mn Fe2 O4 ferrofluids to the living cells have been carried out with BHK-21 cells. In vitro studies showed that threshold biocompatible concentration is dependent on the nature of ferrites and coating. © 2005 American Institute of Physics.