We report a low-cost paper-based diagnostic device (Microfluidic paper based analytic device or muPAD) for diagnosing the biochemical risk factors associated with the onset of Osteoporosis. Photolithography is used to pattern the paper with hydrophilic channels and hydrophobic walls. Briefly, the analytes (serum Calcium and Alkaline Phosphatase) are measured by placing a drop of blood on the sample zone in the device. The serum flows to the test zones containing the immobilized reagents and produces a color reaction. The responses are then compared with the controls and the shift in analyte values from normal indicate normal or diseased state. © 17CBMS-0001.