Microfluidics Large Scale Integration (mLSI) offers low cost, efficient and accessible platform for problems in biology, chemistry, bio-medical applications and environmental monitoring etc. However, the challenges of scalability and cost still hinder its mass adoption. Here, we merge mLSI with lensless imaging and present a miniaturized microfluidic system as a step towards addressing these concerns. Microfluidic circuits containing 3-layered pneumatically controlled Quake valves are fabricated using an improved process. The functioning of valves is confirmed by regulating flow of microspheres in liquid medium. A low cost, easily manufactured, miniature lensless microscope is demonstrated for tracking of these microspheres. © 2019 IEEE.