In this paper, we proposed a method for classification of medical images captured by different sensors (modalities) based on multi-scale wavelet representation using dictionary learning. Wavelet features extracted from an image provide discrimination useful for classification of medical images, namely, diffusion tensor imaging (DTI), magnetic resonance imaging (MRI), magnetic resonance angiography (MRA) and functional magnetic resonance imaging (FRMI). The ability of On-line dictionary learning (ODL) to achieve sparse representation of an image is exploited to develop dictionaries for each class using multi-scale representation (wavelets) feature. An experimental analysis performed on a set of images from the ICBM medical database demonstrates efficacy of the proposed method. © 2014 IEEE.