The present study explores resource recovery from the de-oiled Jatropha curcas seed (DJS) using alkaline pretreatment. Potassium hydroxide (KOH) was utilized for the alkaline hydrolysis at a solid loading rate of 10 % w/v for the kraft lignin (KL) recovery. The process variables optimized were reaction time (6.36–73.63 min), temperature (85.68–156.3 °C), and KOH concentration (0.61–12.38 % w/w). Response surface methodology was used to optimize the process parameters. The maximum amount of KL recovered under optimal conditions was observed to be 89.6 %. The functional group changes in treated and untreated DJS were explored through Fourier transform infrared spectroscopy (FTIR) analysis. 1H NMR and FTIR techniques were employed to characterize recovered lignin. Nitrobenzene oxidation of the lignin was performed to understand the lignin monomer profile. The recovered lignin has an S/G ratio of 0.36. The pretreated DJS biomass can be subjected to enzymatic hydrolysis for enhanced sugar yield. © 2023 Elsevier Ltd