Leptoquarks belong to the possible candidates for explaining various anomalies in flavor physics. Nonetheless, their existence is yet to be confirmed on the experimental side. In this paper we show how zeros of the single-photon tree-level amplitude can be used to extract information as regards leptoquarks in the case of e–γ colliders. A small number of standard model backgrounds keep the signal clean in this kind of colliders. Unlike other colliders, the zeros of the single-photon amplitude here depend on s and on the mass of leptoquark along with its electric charge. We perform a PYTHIA based simulation for reconstructing the leptoquark from its decay products of the first generation and estimating the background with luminosity of 100fb-1. Our analysis is done for all the leptoquarks that can be seen at an e–γ collider with three different masses (70 GeV, 650 GeV and 1 TeV) and three different center of momentum energies (200 GeV, 2 TeV and 3 TeV). The effects of non-monochromatic photons on the zeros of the amplitude under laser backscattering and the equivalent photon approximation have also been addressed. © 2020, The Author(s).