Temporal variations in the chemistry of infiltrating water into the subsurface are known to cause remobilization of colloids from the grain surfaces, thereby increasing the travel distance of the colloidal contaminants. Hence, it is essential to thoroughly understand the transport, deposition, and release mechanisms of colloids in the subsurface, through laboratory experiments and modeling. There are only a few experiments in which the chemistry of inflow water is changed rapidly during colloid transport. Also, although some models have been presented for simulating the effect of transient chemistry on the fate of colloids, there is no consensus in this regard, as the proposed models suffer from shortcomings. In this study, we systematically investigated the effect of temporal variations in ionic strength on the remobilization of deposited colloids in saturated porous media through laboratory column experiments and numerical modeling. Four sets of column experiments were performed, in which we injected carboxylate-modified latex colloids at a given ionic strength for a specified period. After breakthrough of colloids, the ionic strength of inflowing water was decreased in a stepwise manner to 0 mM (DI water). The initial ionic strength values of the four experiments were 100, 50, 25, and 10 mM. We observed partial release of deposited colloids after several steps of ionic strength decrease with significant release observed only when the ionic strength was reduced to below 10 mM. We also found that the fraction of released colloids decreased with increasing value of initial ionic strength of inflow water. We have developed a mathematical model incorporating a novel formulation for ionic strength-dependent deposition and release. The model is found to capture the colloid breakthrough curves reasonably well for all experiments with the same set of parameter values, except the one at the initial ionic strength of 25 mM. © 2022 Elsevier B.V.