We present an analysis of the microfluidic Dean flow fractionation (DFF) method in low aspect-ratio spiral chip for high concentrations (> 106 particles /mL) of sub-micron (1-3 μm) particles. We systematically analyze the particle stream profile (i.e. thickness and distance from the channel wall) as a function of downstream channel length for step-wise increase in the fluid velocity and the flow rate ratio (FRR) of the particles to the sheath fluid. © 2020 CBMS-0001