Accurate diagnosis of Alzheimer's disease (AD) in early stage can control the disease progression. Enlargement of Lateral Ventricles (LV) is one of the significant imaging biomarkers for the differentiation of Alzheimer's conditions. However, segmentation of accurate LV for analysis is still challenging. In this work, an attempt is made to segment LV regions from brain MR images using the UNet++ model. For this, axial scans of the MR images are taken from the publicly available Open Access Series of Imaging Studies (OASIS) Brain dataset. LV-based region of interest is segmented using the UNet++ network. Results show that the proposed approach is able to segment brain regions in Alzheimer's conditions. The UNet++ network model yields the highest dice score of 99.4% and sensitivity of 99.3% in segmenting the LV brain region. Thus, the proposed method could be useful for characterizing Alzheimer's condition. © 2022 The authors and IOS Press.