A B S T R A C T: In depicting the fraught journey of a woman stand-up comedian in the late 1950s and 1960s, The Marvelous Mrs. Maisel portrays stand-up as an ethical medium of truth telling and of sociopolitical protest that challenges systemic hierarchies of gender and sexuality. In this article, I show that stand-up’s foundational subversion is in its professionalism and in its departure from other forms of art. In the show, female stand-up comic Midge Maisel learns to function first as a seasoned professional and then as an ethical crusader who ultimately mounts a two-pronged attack on the sexist underpinnings of American humor and the patriarchal structure of stand-up, substantially influencing the way we receive this liminal genre of cultural expression and reframing it as an empowering vehicle of the marginalized. Copyright © 2023 The Pennsylvania State University, University Park, PA.